Rapid and specific method for evaluating Streptomyces competitive dynamics in complex soil communities.

A Rapid and Specific Method for Quantifying Streptomyces Competitive Dynamics in 1 Complex Soil Communities 2 3 RUNNING TITLE

جداسازی و تأیید مولکولی سریع استرپتومایسس های تولید کننده آنتی بیوتیک استرپتومایسین

Introduction: Streptomyces species are mycelial, aerobic gram-positive bacteria that are isolated from soil and produce a diverse range of antibiotics. Streptomyces griseus produces the antibiotic, streptomycin and forms spores even in a liquid culture. The gene cluster for the production of Streptomycin antibiotic contains strR gene that encodes StrR, a pathway-specific regulator. Then, this p...

Plant monocultures produce more antagonistic soil Streptomyces communities than high-diversity plant communities

Plantesoil feedbacks are important to productivity and plant community dynamics in both natural and managed ecosystems. Among soil bacteria, the Streptomyces possess particularly strong antagonistic activities and inhibit diverse plant pathogens, offering a clear pathway to involvement in plantesoil feedbacks. We hypothesized that feedback effects and the ability of individual host plant specie...

Usefulness of strb1 and 16S rDNA-targeted PCR for detection of Streptomyces spp. in environmental samples.

In this study, we revealed rapid detection of streptomycin-producing Streptomyces spp. by extraction of total soil DNA from 14 soil samples using a modified lysis method followed by PCR amplification ofa genus-specific sequence in the Streptomyces' 16S rDNA gene. DNA band of the expected size (438 bp) was seen with all the samples. Additionally, specific amplification of the streptomycin-coding...

STRUCTURAL DAMAGE PROGNOSIS BY EVALUATING MODAL DATA ORTHOGONALITY USING CHAOTIC IMPERIALIST COMPETITIVE ALGORITHM

Presenting structural damage detection problem as an inverse model-updating approach is one of the well-known methods which can reach to informative features of damages. This paper proposes a model-based method for fault prognosis in engineering structures. A new damage-sensitive cost function is suggested by employing the main concepts of the Modal Assurance Criterion (MAC) on the first severa...